Notes on Genus: Maculavirus

icosahedra

Type member: Grapevine fleck virus

Contents

• Members of this Genus
• General Description
• Morphology
• Electron Micrograph
• Genome
• Genus Genomic Organization
• Genome Map
• Type Member Genomic Organization
• All Known Sequences
• Curated Sequences

General Description

The genus Maculavirus, one of three genera in the family Tymoviridae, is a small group of phloem-limited isometric viruses which are not sap-transmissible and with no known vectors. Phylogenetic analysis indicates some relationship to the other genera in the family but the genome size is larger and members of the genus Tymovirus are beetle-transmitted while those of the genus Marafivirus are transmitted by leafhoppers.

Morphology

Virions isometric (icosahedral), not enveloped, c. 30 nm in diameter.

Genome

Monopartite, linear, single stranded, positive sense RNA approx. 7.5 kb in size. The 3' terminus has a poly(A) tract.

Genus Genomic Organization

Only the type member has been fully sequenced and this is believed to have four ORFs. The largest is a replication protein and the three smaller ones overlap and are probably translated from two or more subgenomic RNAs.

Type Member Genomic Organization

The RNA is translated from four open reading frames:

1	  216 kDa	Replication protein with methyltransferase and polymerase domains
2	24 kDa	Coat protein
3	31 kDa	proline rich; unknown function
4	16 kDa	proline rich; unknown function